Coding

Part:BBa_K3112002:Design

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-09-12)


dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1156


Design Notes

We designed point mutation sequences in primers.


Source

Laboratory

References